Electroporation
Bio-Rad Gene Pulser Xcell Electroporation System
Device Description
It is a flexible, modular electroporation system used to transfect all cell types, from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria and fungi. The system consists of a main unit, two accessory modules, a capacitance extender (CE module), a pulse controller (PC module), and a ShockPod cuvette chamber.
Asst. Prof. Dr. Gamze YEŞİLAY
LAB 214
- Waveform: Exponential or square
- Voltage: 10–3000 V
- Capacitance: 10–500 V, 25–3,275 µF in 25 µF increments
- 500–3,000 V, 10, 25, 50 µF
- Resistance (parallel): 50–1,000 Ω in 50 Ω increments, plus infinity
- Transfection studies in T cells, primary cells, suspension cells, and difficult-to-transfect cells
- Transfection studies in bacteria and fungi
